Staining solution reagent 04-121812

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 45 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Method indicated for simultaneous viewing of DNA and RNA on histological sections. Particularly indicated for highlighting plasma cells and RNA in histological sections and cytological preparations. Result DNA pale green RNA (plasma cells, nucleoli, blasts) pink – red Mast cell granules blue Background contrast turquoise Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate simultaneously DNA and RNA in histologic sections. Recommended to show plasma cells and RNA in histologic sections and cytological preparations. PRINCIPLE In this method, staining is obtained with a mixture of two basic dyes: purified methyl green and pyronin Y. In order to obtain a differential stain, a buffer is added to the solution to reach pH 4,8. If pH is lower than 4,8 a red stain caused by pyronin prevails; if pH is higher, a green-blue stain caused by methyl green prevails. The two dyes are not intrinsically affine to DNA and RNA; their selectivity is a consequence of a definite pH value. This procedure may be difficult; we advise to read warning notes carefully and to work in as a good conditions as possible.