Minimum number of tests that can
be performed
100
Completion time 60 minutes
Shelf life 2 years
Storage conditions 15-25°C
Additional equipment Not required
Application
Method for demonstrating elastic fibers on histological sections, particularly indicated for
vascular pathology
Result
Elastic fibers and nuclei black
Collagen red
Other tissue elements yellow
Product for the préparation of cyto-histological samples for optical microscopy.
To demonstrate elastic fibres in tissue sections. Recommended for vascular pathology.
PRINCIPLE
The method is based on the affinity towards elastic fibres of the hematoxylin with Lugol solution. This is not a spécifie method, other structures such as collagen and muscle tissue might be stained. Therefore, it is essential to differentiate carefully, in order to obtain a sélective marked staining of elastic fibres. Counterstaining with Sirius red picrate solution makes it possible to differentiate the connective tissue.