Staining solution reagent Von Kossa

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 1 hour 25 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment Not required Application Method indicated for viewing calcium ions on histological sections. Result Sites where calcium salts were present black Nuclei red Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate deposits of calcium ions in tissue sections. PRINCIPLE This method is based on a substitution reaction. Tissue is treated with a silver nitrate solution; cationic silver replaces calcium in the original salt thus forming a silver salt that can be showed by a reduction to metallic silver. Reaction is as follows: CaCO3 + AgNO3 = Ag2CO3 + Ca(NO3)2 Ag2CO3 + 2H + = 2Ag + H2O + CO2 Calcium salts liable to react with silver nitrate are carbonate, phosphate, oxalate, sulphate, urate, chloride, sulfocyanide. Since calcium is present in mammalian tissue essentially in the form of calcium carbonate and calcium phosphate, this method is suitable to point it out. To prevent false results caused by the presence of uric acid and urates in the tissue, these salts have been solubilized in a lithium carbonate saturated solution.