Staining solution reagent 04-051802

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 50 minutes + overnight Shelf life 2 years Storage conditions 15-25°C Additional equipment Vertical histology jar with lid Application Combined method for viewing elastic fibers, connective tissue, collagen and nuclei on the same preparation. Van Gieson trichrome is the most commonly used method in association with Weigert staining for elastic fibers. Result Elastic fibers purple - brown Nuclei black Collagen red, in various shades Connective tissue, erythrocytes yellow Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate elastic fibres, connective tissue, collagen and nuclei. PRINCIPLE The method is based on the affinity towards elastic fibres displayed by resorcin fuchsin, a precipitate resulting from a reaction between resorcin and basic fuchsin and ferric chloride. Since this is not a specific method, other structures such as collagen and basal membranes might be stained. Therefore, it is essential to differentiate carefully in order to obtain a selective marked staining of elastic fibres. Weigert’s “long method” differs from “quick method” in both staining mixture and procedure; it is more selective because staining mixture is less concentrated and incubation times are very long. Counterstaining with Van Gieson trichrome stain makes it possible to differentiate collagen from connective tissue showing nuclei at the same time.