Bio-Optica provides operators with a complete range of nuclear stains; all solutions are
stable and yield excellent cellular details.
Similar to Carazzi’s hemalum
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l.
HDPE cap. Tamper evident cap.
Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water
and alcohol.
Expected aim Product for the preparation of cyto-histological samples for optical microscopy.
Application Nuclear stain for histology and for staining according to Papanicolaou.
Principle In Gill’s Hematoxylin the active chemical species is the complex formed by hematein (hematoxylin oxidized by potassium iodate) with aluminum potassium sulfate. This complex has a positive charge and is therefore able to bind to anionic sites present in the chromatin histone proteins.
Method
1) Sections to water
2) Gill’s hematoxylin n. 1, 5 minutes
3) Colour change in tap water, 5 minutes
4) Acqueous 1% eosin solution, 5 minutes
5) Tap water, 5 minutes
6) Dehydrate
7) Clearing agent and mount
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.
In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities