Staining solution reagent 05-M12005

for histology

Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Reagent used with May Grünwald for the staining of different kind of cells in blood and bone marrow smears. This staining is also used to highlight the Helicobacter pylori in histology. For the execution of the staining method is required the use of May Grünwald solution. Principle Two dyes are used one after the other: - May Grünwald solution, consisting of eosin-methylene blue, stains nuclei blue and basophil cytoplasm in pinkish red; - Giemsa solution, complex consisting of methylene blue chloride, eosin-methylene blue and azure II eosinate, improves the intensity of nuclear staining and the capacity to show selectively cellular structures. To appreciate results always remember two factors: pH of washing waters and dilution buffer have a strong influence on final colour chart; intensity of stain may vary according to differentiation time. Method 1) Air dry smears 2) May Gruenwald solution 5 minutes 3) Wash in tap water 1 minutes 4) Giemsa working solution* 15 minutes 5) Wash in tap water 1-2 minutes