Enzyme reagent Atlas H Minus

DNA polymerasereverse transcriptasefor RNA analysis

cDNA synthesis – RNA analysis by primer extension – DNA labeling DESCRIPTION: Atlas H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity. Reagents Provided: Atlas H Minus M-MLV Reverse Transcriptase 5x RT Reaction Buffer(with added DTT) – 250 mM Tris-HCl (pH8.3), 375 mM KCl, 15 mM MgCl2, 50 mM DTT. 5 x RT Reaction Buffer (DTT free) – 250 mM Tris-HCl (pH8,3), 375 mM KCl, 15 mM MgCl2 100 mM DTT Shipping and Storage conditions: Shipping at room temperature has no detrimental effects on the quality of this reagent.