Solution reagent Titan HotTaq Probe Universal

DNA polymerasefor microbial contaminationfor DNA quantification

DNA/LNA hydrolysis probe-based assays Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination DESCRIPTION: Titan HotTaq Probe qPCR Universal Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. The qPCR Mix contains optimized components and Titan HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. Titan HotTaq Probe qPCR Universal Mix is optimized for DNA/LNA hydrolysis probes based on the 5′ flap endonuclease activity. Titan HotTaq DNA polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Composition: Titan HotTaq DNA polymerase Titan HotTaqProbe qPCR Universal buffer 15 mM MgCl2 1x PCR solution – 3 mM MgCl2 dNTPs, including dUTP. The mix allows UNG treatment to prevent carryover contamination from previous runs.