Stain reagent Atlas ClearSight

nucleic acidfor researchfor electrophoresis

APPLICATIONS: – Non-carcinogenic alternative to ethidium bromide. – Excellent for nucleic acid electrophoresis and purification applications. – For visualizing ribonucleic acids in agarose gels. Comments: This product is supplied for research use only. DESCRIPTION: Atlas ClearSight DNA Stain is a new, non-carcinogenic, sensitive and convenient stain to use instead of highly carcinogenic ethidium bromide stain (EtBr) for detecting nucleic acid in agarose gels (Figure 1). It has two secondary fluorescence excitation peaks (~270 nm; ~295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to EtBr when bound to DNA – at ~ 530 nm. PROTOCOL: Precasting: Prepare 100 ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible. Add 4-6 µl of Atlas ClearSight DNA Stain to the cooled gel solution (60-70ºC), mix it gently and pour into the gel tray (for higher sensitivity, the gel should be less than 0.5 cm thick). When the gel is solid, load the samples and perform electrophoresis. Detect the bands under UV ( yellow or green gelatin or cellophane filters, preferably).