DNA polymerase reagent Titan Taq

for researchfor PCR

Titan Taq DNA Polymerase APPLICATIONS: – Suited for a wide range of PCR assays. – Primer extension. – TA cloning. DESCRIPTION: Titan Taq DNA Polymerase is highly processive, thermostable DNA polymerase (Fig.1). The enzyme has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. The enzyme has “extendase activity” allowing TA cloning. Concentration: 5 units/µl Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74ºC. Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Quality control: The enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS/PAGE – 95 kD band, >98% pure. Activity and stability tested via thermocycling. The error rate per nucleotide per cycle is ~2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95°C is 1.5 hours. Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Supplied with: 10 x Reaction Buffer (Mg2+ free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH4)2SO4, 0.2% w/v Tween-20. 25 mM MgCl2