DNA polymerase reagent Atlas HotTaq

for researchfor PCR

Atlas HotTaq DNA Polymerase APPLICATIONS: – Polymerase chain reaction setup at room temperature. – Effective incorporation of modified nucleotides. DESCRIPTION: Atlas HotTaq DNA Polymerase catalyzes 5´ to 3´synthesis of DNA. The enzyme has been proved not having the 3´ to 5´ exonuclease activity. Prior the first PCR step the Atlas HotTaq DNA Polymerase should be activated by 15 minute incubation at 95-97°C. Concentration: 10 units/µl Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C. Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase. Quality control: Free of detectable, non-specific nucleases. Activity and stability tested at 20, 30 and 40 cycles of PCR reaction at 95°C. Tested for the absence of human DNA contamination by PCR with Alu-specific primers. Supplied with: Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40. Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% Tween 20. Atlas 25 mM MgCl2 Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Guaranteed stable for 12 months when properly stored. Comments: This product is supplied for research use only.