DNA polymerase reagent Atlas Taq

for researchfor PCRfor DNA sequencing

Atlas Taq DNA Polymerase APPLICATIONS: – Polymerase chain reaction (PCR). – Primer extension. – DNA sequencing. DESCRIPTION: Atlas Taq DNA Polymerase is highly thermostable DNA polymerase (Fig.1). The enzyme catalyzes 5’ to 3’ synthesis of DNA, but lacks 3’ to 5’ exonuclease activity. Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C. Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase. Quality control: Free of detectable, non-specific nucleases. Supplied with: Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40. Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% Tween 20. Atlas 25 mM MgCl2 Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Guaranteed stable for 12 months when properly stored. Comments: This product is supplied for research use only.