Solution reagent Atlas RedTaq

DNA polymerasefor researchfor PCR

– Ready-to-use mix reduces experimental variability. – Reduced pipetting steps, thereby less time-consuming set-up of PCR. – Set-up at room temperature. – Visualization of complete reaction mixing. – Direct loading of samples following amplification. DESCRIPTION: Atlas RedTaq 2x PCR Mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes high-quality recombinant Atlas HotTaq DNA Polymerase, nucleotides in a specifically optimized buffer formulation and MgCl2 with final concentration of 2 mM. Added inert dye will provide visual confirmation that the enzyme has been added and that the proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition. For reaction set-up add the PCR Mix (25 µl) to the primers, template and water (is provided in the set; total volume of 50 µl). Quantity: The mixture contains 100 units of Atlas RedTaq Hot Thermostable DNA Polymerase. It is sufficient for 100 reactions of 20 µl or 40 reactions of 50 µl. Guaranteed stable for 1 year when properly stored. Comments: This product is supplied for research use only.