Solution reagent Titan HotTaq

DNA polymerasefor researchfor PCR

– Hot Start PCR – Proofreading DESCRIPTION: Titan HotTaq Power Mix, Ready to Load is a premixed ready to load solution containing: all reagents required for PCR (except template, primers and water), compound needed for direct loading onto agarose gel and two tracking dyes to follow the electrophoresis. Titan HotTaq Power Mix, Ready to Load contains two enzymes – Titan HotTaq DNA polymerase and a proofreading polymerase. These enzymes together have both the 5′ to 3′ exonuclease activity as well as the 3′ to 5′ proofreading activity. Titan HotTaq Power Mix, Ready to Load is recommended to use in any PCR application that will be visualized by agarose gel electrophoresis and AtlasSight DNA Stain or Ethidium bromide staining. Composition: Titan HotTaq DNA Polymerase Proofreading enzyme 5x Reaction Buffer 12.5 mM MgCl2 (1x PCR solution – 2.5 mM MgCl2) 2 mM dNTPs of each (1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP) BSA Blue dye (Migration equivalent to 3.5-4.5 kb DNA fragment) Yellow dye (Migration rate in excess of primers in 1% agarose gel: <35-45 bp) Compound that increases sample density for direct loading Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of Titan HotTaq Power Mix, Ready to Load. Comments: This product is supplied for research use only.