Enzyme reagent kit 300 series

buffer solutionfor PCRNGS

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes. The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most of DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time Some genomic regions are very difficult to be covered evenly and usually result in very low coverage rate or gap in these regions. Typical difficult regions are: • with high GC contents • have secondary structures: mainly due to repeat sequences • the worst cases: have both high GC contents and repeated sequences. Example: human TERT gene is one of the most difficult regions as shown above. NGS data showed that BioDynami kit has the best performance to cover the extremely difficult human TERT gene region. Three index types are available for the illumina platform kits: Non-index : Libraries do not have index. Index : A unique barcode sequence with 6 bases has been included in each of the index primers.