Enzyme reagent kit 3 series

buffer solutionfor PCRNGS

The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation. The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic approach (for example, BioDynami DNA fragmentation enzymes, and mechanical approaches such as sonication and nebulization etc. Kit advantages: Fast protocol The hands-on time is only 10 minutes The total protocol time is around 1.5 hours Simple procedure Ready-to-use master mix makes it simple for reaction setup Less reaction components Less magnetic beads required for cleanup steps: Save the cost more than 50% Low input DNA amount: Starts from 1 ng of DNA Three index types are available for the NGS Low Input DNA Library Prep Kit of illumina platform: Non-index : Libraries do not have index. Index: Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify the low input DNA libraries. Library multiplexing up to 48 samples is possible. Unique dual index: Library multiplexing up to 96 low input DNA libraries is possible with unique dual indexes with our Four-Base Difference Index System.