Enzyme reagent 30052

buffer solutionNGSfor DNA library preparation

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible. The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C. Kit features: Fast: 1-hour library construction from intact genomic DNA to NGS library Total time: 1 hr Hands-on time: 5 min Intact genomic DNA can be used directly as input; DNA fragmentation is not required. Works with both EDTA-free DNA samples and DNA samples in TE buffer Simple workflow Three steps Only one beads purification step Guaranteed quality: high library conversion efficiency based on our chemistry