FFPE tissues reagent kit 3 series

enzymebuffer solutionNGS

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible. FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples. Thus, the extraction of high quality DNA from FFPE samples is often a challenge. Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation. As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples. Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform: Non-index : Libraries do not have index. Index : Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples. Index information can be downloaded here. Unique dual index: FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system.