The kits (listed below) were developed to capture the MHC locus from whole genome NGS libraries based on the CATCH-Seq technology. Moreover, kits combining NGS library prep and MHC locus capture are also available. The integration of library prep and MHC capture not only simplifies the procedure, but also enhances the capture efficiency. Comprehensive sequencing of the selected locus with our kit make it possible to detect SNPs, indels, and structural variants that are not covered by other MHC targeted sequencing reagents.
Features:
Integration of library prep with capture or capture alone
Simplifies the procedure
Enhances the capture efficiency
Multiple choices of targeted MHC regions
Kits available for the following regions
Full 3.8 MB of MHC region
2.1 MB Class I region
0.7 MB Class II region
1.1 MB Class III region
190 kb MHC core regions (22 MHC genes)
Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond.
Easy detection of SNPs, indels, and structural variants
The only reagent provides intact sequence information
Low cost
Sample multiplexing: up to 96 samples can be pooled together to further reduces the cost
Index: For kits with 24 reactions. Sample multiplexing up to 24 libraries is possible. Each of the index primers (#1~#24) has a unique 6-base index sequence that can be used to identify libraries
Unique dual index: For kits with 96 and 384 reactions. Sample multiplexing up to 96 libraries is possible