Enzyme reagent kit 3 series

buffer solutionNGSfor RNA library preparation

The RNA Seq Library Prep Kit was developed for construction of high quality NGS libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes. Three index types are available for the illumina platform kits: Non-index : Libraries do not have index. Index : A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here. Unique dual index : RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here. Indexes are available for the MGI platform kits . Features Quick protocol Libraries will be ready in 2 hours Hands-on time is only ~10 minutes Guaranteed quality High yield Uniform coverage of transcripts Simple workflow Input purified RNA amount: From 3 ng to 100 ng