Water analysis detection kit BIK-Q-W025

for fishvirustissue

This kit is suitable for the specific detection of pathogenic Streptococcus iniae septicaemia virus in fish fry or viscera tissue samples such as heart, intestine, spleen and kidney. Principle: This kit designed primers with Streptococcus iniae septicaemia virus specific fragment to probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Streptococcus iniae septicaemia virus in the totally closed reaction system was realized by fluorescence PCR instrument. Sample requirements: 1. Sample type Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples. 2. Sample collection and transportation Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw. 3. Sample preservation It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃. Quality control: Negative control: No obvious amplification curve of FAM detection channel; Positive control: FAM channel showed obvious amplification curve, Ct value ≤30; The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.