This kit is used for diagnosis, monitoring and epidemiological investigation of porcine circovirus type 2, and is suitable for specific detection of porcine circovirus type 2 (PCV2) in pig serum, feces, semen, viscera and other tissue samples.
Principle:
This kit selection of porcine circovirus virus type 2 conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The porcine circovirus type 2 nucleic acid was detected by fluorescence PCR instrument in a completely closed reaction system.
Sample requirements:
1. Sample type:
Pig serum, feces, semen, viscera and other tissue samples
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.