This kit is suitable for the specific detection of leptospirosis (Lep) and Toxoplasma (TOX) in EDTA whole blood samples of cats and dogs.
Principle:
This kit selects the leptospirosis (Lep) and Toxoplasma (TOX) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acids of leptospirosis and Toxoplasma gondii were detected by fluorescence PCR apparatus in a completely closed
Sample requirements:
1. Sample type
Feline/dog EDTA whole blood samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.