Feline leukemia test kit BIK-Q-P035

AIDSfor felineswhole blood

This kit is suitable for the specific detection of feline leukemia virus (FeLV) and feline AIDS virus (FIV) in EDTA whole blood sample. Principle: Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose feline leukemia virus (FeLV) and feline AIDS virus (FIV) gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to realize nucleic acid detection of f feline leukemia virus (FeLV) and feline AIDS virus (FIV) virus in the completely closed reaction system. Sample requirements: 1. Sample type EDTA whole blood 2. Sample collection and transportation Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw. 3. Sample preservation It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.