Solution reagent 02-023-1A
salt solutionDPBSfor cell culture

solution reagent
solution reagent
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Characteristics

Type
solution, salt solution, DPBS
Applications
for cell culture
Format
liquid
Tested parameter
calcium, magnesium

Description

Dulbecco's Phosphate Buffered Saline (DPBS) is intended to provide a buffer system for maintaining cell culture media in the physiological range of 7.2– 7.6. It generally serves as an isotonic saline solution or buffer for washing cells and tissues. It's utility in maintaining mammalian cells when a chemically-defined, balanced salt solution provides the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro for a limited period of time. Biological Industries offers a range of DPBS formulations. DPBS without calcium (Ca2+) and magnesium (Mg2+) is often used in trypsin solutions. DPBS without calcium and magnesium is used in the dissociation process to wash and resuspend cells when the presence of calcium and magnesium may inhibit trypsin activity. This DPBS is modified as follows: Without: Calcium Magnesium The complete formulation is available. Specifications Phenol Red Indicator - No Phenol Red Storage Conditions - AMB Sodium Bicarbonate Buffer - No Sodium Bicarbonate Inorganic Salts - No Calcium Chloride, No Magnesium Chloride

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