Solution reagent NutriCoat™

for researchclinicalliquid

Cost-effective, efficient, standardized supplement designed for the attachment of human mesenchymal stem cells under serum-free and xeno-free culture conditions. Product Overview: NutriCoat™ is a defined substrate based on clinical-grade human Fibrinogen, designed for the attachment of human mesenchymal stem cells (hMSC) in serum-free and xeno-free culture systems. NutriCoat™ Attachment Solution is optimal for adherence of hMSC’s from multiple sources (e.g. AT, CT, BM, DP) when cells are cultured with MSC NutriStem® XF Medium. The substrate supports long term culturing of hMSC, as well as their enumeration using the colony forming unit-fibroblast (CFU-F) assay under SF and XF culture conditions. NutriCoat™ is a ready-to-use solution store at RT and ideal for cell therapy research applications. Features Defined substrate containing clinical grade human Fibrinogen (xeno-free) Easy-to-use stock solution for easy handling cGMP Manufactured Suitable for both hMSC expansion and differentiation Supports long-term multi-potency of hMSC Validated for multiple sources of human MSC culture Allows quick transition from research to clinical applications Specifications QTY - 1.5 mL/vial quality control - Each lot is tested for sterility as well as its biological performance (the ability to promote the attachment and spreading of hMSC in serum-free medium). Instructions for Use Dilute the solution 1:500 in saline and gently mix using a pipette. Coat the culture dish with the 1:500 solution. The volume should be adequate for covering the surface of the culture ware. Use table 1 for the recommended volumes. (e.g. per 1 well of 12w/p add 2μl of NutriCoat™ in 1 ml saline)