Enzyme reagent kit AccuRapid™

for researchfor PCR

Bioneer’s AccuRapid™ Cloning Kit allows accurate and rapid cloning of 1 ~ 3 inserts (PCR products) into a linearized vector by recognizing both ends of PCR amplified inserts and their complementary sequences for 18 ~ 21 bp ends of linearized vectors and connecting them. Unlike the conventional method of using ligation method after the treatment with restriction enzymes, inserts to be cloned can be rapidly through PCR and accurately in the desired position.(Amplification can be done faster through the use of our world's fastest PCR instrument, ramping rate 9.5°C/sec, AllInOneCycler™.) ※This product is shipped in dry ice. Features and Benefits Fast & Accurate cloning Cloning with 30 minute reaction Simplified protocol Use of a primer containing a sequence complements to 18-21 bp both ends of the vector to amplify the insert and clone it in the desired direction without restriction enzyme treatment Simultaneous cloning Simultaneous cloning of 1~3 insert fragments in the desired sequence using both ends complementary sequences of each fragment. User-friendly customization Easily customize cloning designs and enable vectors on the desired form. ▶ Experimental Method 1Linearized Vector Preparation Before using AccuRapid ™ Cloning Kit, it is crucial to completely linearize the vectors using PCR or restriction enzymes. Linearized vectors have higher cloning efficiency, with reduced chances of having colonies of circular vectors without the inserts while increasing those with them. * To increase the cloning efficiency, it is recommended to check whether the vectors are linearized by electrophoresis before proceeding to the next step.