Solution reagent kit AccuOligo®

for mutation detection

This product consists of 2 sets of 3,308 primers. It is used to confirm the quality of S. pombe haploid deletion mutants ver3.0 by PCR. It consists of mixture of a specific primer for the target gene and a common primer for the KanMX module, a deletion cassette. so you can easily prepare PCR mixture by one pipetting. Features and Benefits Deletion mutants of each S.pombe gene Similar physiological process with mammalian cells Human cancer-related genes with over 30% of homology Rapid cell cycle(~3h) with simplified analysis of molecular biological mechanism and pathway Phenotype analysis possible with it's recessive mutant type Unknown genomic function analysis through functional complementation Drug target screening at the genomic level in living cells available Overview S. pombe (Schizosaccharomyces pombe) Genome-wide Deletion Mutant Library was developed in collaboration with BIONEER, Korea Research Institute of Bioscience and Biotechnology (KRIBB) and Cancer Research UK. BIONEER is the only institute with exclusive business license. It is a powerful research tool for gene function analysis, drug target identification, validation studies, and global cell function studies. It can also be used for gene function analysis and drug screening, such as gene expression and synthetic lethal analysis. S. pombe Deletion Mutant Library was produced by homologous recombination, replacing the target gene with a selective cassette containing KanMX4