DNA polymerase reagent kit E-20 Series

Taq DNA polymerasedilution bufferfor molecular biology

Taq DNA polymerase is a thermostable polymerase derived from the gene of Thermus aquaticus YT1 expressed and purified from Escherichia coli . ※This product is shipped in dry ice. Features and Benefits Efficiency & Sensitivity High efficiency and sensitivity to the templates Optimized buffer delivery Stable PCR reaction by providing buffers optimized for the enzyme Reproducibility Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system Application Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye Gene expression profiling Microbial & viral pathogen detection Specifications 5' to 3' exonuclease activity Yes 3' to 5' exonuclease activity No 3' – A overhang Yes Fragment size Up to 10 kb Components • 10X reaction buffer with (or without) MgCl2: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0 • Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0 • 10 mM dNTPs mix: 2.5 mM of each dNTP ▶ Concentration 500 units (5 U/μl) ▶ Storage conditions 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0 ▶ Unit Definition One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.