AccuPower® PCR PreMix (with UDG) helps to minimize carryover contamination, which may cause severe problems in PCR during clinical dignosis. Although PCR is one of the most sensitive and rapid tests, contamination by amplified PCR products from previous tests may lead to false postive results. However, this product has overcome this problem through the application of Uracil DNA Glycosylase system.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Features and Benefits
Carryover Contamination Prevention
Minimized false positives caused by a carryover contamination through application of uracil DNA glycosylase system
User-friendly
Simplified procedure by packaging each of the tubes with sufficient amounts of reactants required for one run, allowing anyone to perform PCR tests simply by adding template DNA and primers
Stability
Stabilizer included in the PCR reaction mixture to keep stable enzymatic activities for up to 2 years if stored at -20 ° C.
Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables by a mass production under ISO 9001 quality system
Application
• Conventional PCR
• Primer extension
• TA cloning
• Gene sequencing
• Molecular diagnosis
Specifications
Enzyme Top DNA polymerase
5' to 3' exonuclease activity No
3' to 5' exonuclease activity No