AccuPower® GoldHotStart Taq PCR PreMix applies GoldHotStart Taq DNA polymerase to not only enhance specificity and PCR amplification, but also reduce non-specific reactions such as mis-priming and primer-dimer formation that may occur at a low temperature. In addition, the components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run.
※ Master Mix product is shipped in dry ice
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
Features and Benefits
Specificity
Minimized generation of non-specific amplification products in the zero cycle by GoldHotStart Taq DNA polymerase and accurate amplification of correct target seqences by activated HotStart Taq polymerase
Sensitivity
Excellent sensitivity and amplification efficiency with mimimized non-specific reactions by detecting even a trace amount of samples
User-friendly
Simplified procedure by requiring only the addition of template DNA, primer sets, and D.W., with all necessary components and DNA polymerases included and mixed in each of the tubes packaged with sufficient amount for 1 PCR run, along with tracking dye and sedimentation agents also included for electrophoresis not requiring the addition of sample loading buffer