AccuPower® ProFi Taq PCR PreMix uses ProFi Taq DNA polymerase developed by Bioneer which allows accurate amplification of long template DNA with high fidelity and efficiency. As the components necessary for PCR such as ProFi Taq DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run, by simply adding the template DNA and primers, a high-performance PCR product can be obtained.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
Features and Benefits
High Efficiency, High Sensitivity PCR Amplification
Guaranteed accurate PCR products with the excellent sensitivity and the best amplification efficiency
Long Range PCR
Effective amplification of large genomic DNA fragments up to 20 kb of human DNA
Convenience
Simplified procedure only requiring the addition of templates and primers with vaccum-dried components
Stability
Enhanced stability allowing maintaince of stable enzymatic activities after long-term storage by including a stabilizer and dried in the PCR reaction mixture
Reproducibility
Minimized deviation between lots through a mass production under ISO 9001 quality system, allowing reproducible results even for replicates tested repeatedly under same conditions and variables
Application
• Primer extension
• long-range amplification from genomic DNA