AccuPower® RocketPlex RT-PCR PreMix is designed for continuous cDNA synthesis and PCR of two or more target genes. Maximum of 10 target RNAs can undergo one-step RT-PCR simultaneously with excellent thermal stability through the application of RocketScript™ RTase, making it also suitable for the cDNA synthesis of secondary RNA structures with increased reaction specificity using HotStart Taq DNA Polymerase. Furthermore, all components crucial for RT-PCR and electrophoresis, including a tracking dye and a sedimentation agent, are each packaged with amounts sufficient for a single run to prevent cross-contaminations from the repetitive use of master mix.
※This product is shipped in dry ice.
Features and Benefits
Superior Multiplex Performance
Simultaneous cDNA synthesis and amplification up to 10 target genes just by adding their primers in a single tube
Single and Multiplex RT-PCR
Specificity
HotStart Top DNA polymerase for increasing the specificity of the PCR reaction.
User-friendly
Amplification of various targets easily in a single tube with one reaction which can be used by anyone
Application
• Multiplex RT-PCR
• Low copy detection
• Gene-expression analysis
Specifications
5' to 3' exonuclease No
3' to 5' exonuclease No
3' – A overhang Yes
Fragment Size Up to 1 kb