Enzyme reagent kit AccuPower® RocketScript™

for RT-PCRgenetic

AccuPower® RocketScript™ RT PreMix, RNase H Minus uses RocketScript™ RTase and RNase H Minus developed by Bioneer independently. By using unique genetic engineering technology, it is useful for cDNA synthesis of long target RNA by removing RNase H activity. It is excellent in extensibility and sensitivity, and can successfully synthesize cDNA even in 1 pg human total RNA. It also contains all the components necessary for cDNA synthesis, so RT-reactions can be performed directly by adding RNA to be amplified and D.W. ※This product is shipped in dry ice. Features and Benefits Rapid Synthesis Short-Time Synthesis : 9 kb synthesis possible with 10 minute RT reaction Time-efficient and economical synthesis of cDNA from secondary structure RNA with RocketScript™ RNase having excellent thermal stability capable of being active at even 70 ℃ No RNase H activity Useful for cDNA synthesis up to 12.5 kb from long target RNA using enzymes developed by proprietary genetic engineering technology Sensitivity High sensitivity and effective synthesis of cDNA even at scarce amount of 1 pg of human total RNA. Convienance Synthesis of cDNA samples difficult to be done using traditional methods, such as those made with long size RNA (9 kb), possible only within 10 minutes Reproducibility Minimized deviation between lots, then supplied as uniform quality product to provide reproducible experimental results by mass production under ISO 9001 quality system Application • First-strand synthesis of cDNA from RNA molecules (RT) • Random priming reaction • cDNA library construction • Probe labeling • mRNA 5‘-end mapping by primer extension analysis • PCR • Real-time qPCR