Reverse transcriptase reagent kit Rocketscript™

for RT-PCRfor real-time PCR

This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs. ※This product is shipped in dry ice. Features and Benefits Long cDNA Synthesis Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H Thermostability Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products Sensitivity Excellent amplification sensitivity even with a trace amount of 1 pg RNA Reproducibility Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system Application Gene synthesis First-strand synthesis of cDNA from RNA molecules (reverse transcription) Random priming reactions Library construction Probe labeling mRNA 5’-end mapping by primer extension analysis Real-time PCR RT-PCR Specifications DNase activity No RNase acitivity No RNase H activity No Fragment size ~12.5 kb Components • 5X Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.3) • 100 mM DTT • 10 mM dNTPs mix: 2.5 mM of each dNTP Concentration 10,000 Unit (200 U/μl) Storage Conditions 50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers. Storage Temperature -20°C Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmole of deoxynucleotide into acid-precipitable material in 10 min at 37℃ using poly A, oligo dT as template primer