BQC ORAC Assay Kit is based on the inhibition of fluorescein oxidation by peroxyl radical in the presence of antioxidants. Peroxyl radical (ROO·) is produced by a free radical initiator (AAPH: 2,2’-azobis-2- methylpropanimidamide dihydrochloride) which produces a fluorescent quenching of fluorescein over time. The presence of antioxidants inhibits the free radical damage to fluorescein, resulting in a preservation of the fluorescent signal.
This kit is a powerful tool to measure the antioxidant capacity of biological samples, cell lysates, tissue homogenates, food, and beverages.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: -20ºC,and 4ºC
Assay time: 100 minutes
Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)
Necessary material: 96 well-plate spectrophotometer