CUPRAC reagent implies oxidation of an antioxidant with a leading thiol group like for example glutathione(GSH), generating a coloured product measurable at 450nm in a spectrophotometer. This reaction takes place on physiological pH (or neutral pH, near pH 7). The chelation with neocuproin enables a fastened reaction by elevating the redox potential of the reagent.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: -20ºC, and 4ºC
Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)
Necessary material: 96 well-plate spectrophotometer