The Polyphenol quantification assay kit has been used as a measure of total phenols in natural products. Its basic mechanism is an oxidation/reduction reaction. The original F-C assay used the carbonate buffer for pH adjustment. Additionally, the end-point of the reaction after 120 min at room temperature, which makes difficult its implementation for routine analysis.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: Room temperature
Reagents: Reagent A, Reagent B, Reagent C, and Standard (Gallic acid)
Necessary material: 96 well-plate spectrophotometer