Cellular biology assay kit KP06003

cellfluorescence

2’-7’dichlorofluorescin diacetate (DCFH-DA) is a cell-permeant reagent fluorogenic dye that measures hydroxyl, peroxyl, and other ROS activity in the cell. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF). Then, DCF can be detected by fluorimetry, flow cytometry or fluorescence microscopy, with maximum excitation and emission spectra of 495 nm and 529 nm, respectively. DCFH-DA probe is a READY TO USE probe suitable for: Flow cytometer Microscopy Fluorimeter Additional information Sizes: 250, 500 and 1000 tests Expiry date: 1 year Storage: -20 ºC Reagents: Reagent A, Reagent B, and Reagent C