Superoxide dismutase assay kit KB03011

for oxidative stresshydrogen peroxideuric acid

Superoxide dismutases (SOD) are metalloenzymes that catalyze the conversion (dismutation) of the superoxide anion (O2•−) into molecular oxygen (O2) and hydrogen peroxide (H2O2), playing a key role in the cellular antioxidant defense system. BQC SOD Activity Assay Kit is based on the reduction of the water-soluble tetrazolium salt [WST-1, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt] to the water-soluble formazan dye (WST-1 formazan, λ = 450 nm) by superoxide radicals (O2•−). In the presence of SOD, a decrease in the rate of WST-1 formazan generation is produced due to the dismutation of the superoxide radical (O2•−) catalyzed by the enzyme. Since the generation of WST-1 formazan is proportional to the amount of superoxide radical (O2•−), the SOD activity as an inhibition activity can be quantified by measuring the decrease in the formation of this compound at 450 nm. Additional information Sizes: 100,200 and 400 tests Expiry date: 1 year Storage: 4ºC Reagents: Reagent A, Reagent B, Reagent C, and Reagent D Necessary material: 96 well-plate spectrophotometer