Nitrite assay kit KB03009

for oxidative stressfor molecular biology

BQC Nitrite Assay Kit is a fast and simple method for the determination of nitrite in a wide variety of samples. Nitric oxide (NO) is an important signalling molecule that participates in virtually every cellular and organ function in the body. NO is a gaseous free radical with a short half-life in vivo. Therefore, the levels of the more stable NO metabolites, nitrite (NO2-) and nitrate (NO3-), are used for the indirect determination of NO. Biological NO is mainly synthetized from L-arginine by nitric oxide synthases (NOS). NO at physiologic levels has different beneficial functions (e.g. antimicrobial, antioxidant, neurotransmitter, etc.). In contrast, high levels of NO and its derivatives (e.g. peroxynitrite) are cytotoxic and produces oxidative stress. Dysregulation of NO signalling pathways is associated with the pathogenesis of cardiovascular disorders and inflammatory diseases. Additional information Sizes: 100,200 and 400 tests Expiry date: 1 year Storage: 4ºC Reagents: Reagent A, Reagent B, and Standard (NaNO2) Necessary material: 96 well-plate spectrophotometer