Assay kit for the quantification of thiols KB03007

for oxidative stressbiologicalfor molecular biology

Thiol Quantification Assay Kit measures the reduced and oxidized thiol groups in biological samples using Ellman´s Reagent. In the assay, Ellman´s Reagent, reacts with GSH (not GSSG), generating a colorimetric signal at 412 nm, so GSH can be specifically quantified. In addition, the assay also allows the measurement of disulfides in the sample, thanks to NaBH4, which reduces them to thiols. GSSG is calculated by subtraction of GSH to GSH + GSSG. Additional information Sizes: 100,200 and 400 tests Expiry date: 1 year Storage: 4ºC and room temperature Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, and Standard (GSH) Necessary material: 96 well-plate spectrophotometer