Oxidative stress assay kit KB03010

nitritenitratefor molecular biology

Nitrate/Nitrite Determination Assay Kit is recommended for the determination of nitrite and nitrate. BQC Nitrite/Nitrate Assay Kit is based on the Griess method. Nitrite reacts with Griess reagent to produce an azo compound that can be spectrophotometrically quantified at 540 nm. Nitrate is converted to nitrite by the enzyme nitrate reductase. Total nitrite (NO2-) can be therefore determined following the two step protocol: enzymatic reduction + Griess reaction. Nitrite (NO2-) concentration naturally present in the sample can be directly quantify using the Griees reaction. Nitrate (NO3-) concentration can be calculated by subtracting nitrite concentration naturally present in the sample from the total nitrite concentration. Additional information Sizes: 100,200 and 400 tests Expiry date: 1 year Storage: -20ºC, and 4ºC Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, Reagent F and Standard (NaNO2) Necessary material: 96 well-plate spectrophotometer