A solution of DMPD at an acidic pH, in the presence of a suitable oxidant solution, can form a stable and colored radical cation (DMPD•+) that shows maximum absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce discoloration of the solution which is proportional to their amount. This reaction is rapid and the endpoint, which is stable, corresponds to a measure of the antioxidative efficiency.
This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: -20ºC, 4ºC and room temperature
Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)
Necessary material: 96 well-plate spectrophotometer