Laboratory bioreactor RTS-8 Plus

cell cultivationfor scientific researchsingle-use

RTS-8 plus is a personal bioreactor that utilizes patented Reverse-Spin® technology that applies non-invasive, mechanically driven, low energy consumption, innovative type of agitation where cell suspension is mixed by the single-use falcon bioreactor tube rotation around its axis with a change of direction of rotation motion resulting in highly efficient mixing and oxygenation for aerobic cultivation. Combined with a near-infrared, fluorescence and luminescence measurement systems, it is possible to register cell growth kinetics, pH and O2 non-invasively in real time. For pH and O2, innovative single-use sensor spots are used inside the tubes. Although O2 supply is one of the major issues in the cultivation of aerobic organisms, especially in oxygen limited conditions, adequate methods for real monitoring of dissolved oxygen were missing, and sufficient O2 supply was usually assumed. Innovative non-invasive oxygen sensors integrated in falcon tubes now enable online oxygen monitoring and give new insights into metabolic activities. The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Cultivation vessels which are sensor limited are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used lacking high data density and interfering with growth. Non-invasive real time pH measurement provides new insights into metabolic activity and changes in metabolic pathways.