Staining solution reagent CyTRAK Orange™

dyefor research

CYTRAK Orange™ is a novel orange fluorescent dye related to DRAQ5™ that stains both nucleus and cytoplasm. It is water soluble and membrane permeant and can be used in LIVE cells in combination with other common fluors, especially GFP fusions, FITC-labelled antibodies and far-red dyes. Why CyTRAK Orange™? CyTRAK Orange™ co-excites at 488 nm with eGFP, FITC and Cy2 flurophores avoiding the need for laser alignment in confocal instruments when this combination is used. CyTRAK Orange™ has an emission profile that permits the simultaneous acquisition of commonly used "green" fluorphore signals (e.g. eGFP, FITC and Cy2) AND far-red FRET dyes (e.g. PE- and APC- Cy7) combined with discrimination of both the nuclear and cytoplasmic compartments. A separate series of parameters can be detected with red-exciting fluorphores such as APC, APC-Cy7 since CyTRAK Orange™ is not excited at 633 or 647 nm. Wide platform compatibility - blue/green excited (Exmax 510 nm) GFP/FITC compatible - orange emission (Emmax 615 nm) Multi-colour compatible - no UV /red excitation Compartments: 1 dye - differential nucl:cyto staining Demarks cell boundaries - even staining of the cytoplasm Allows assay development - works in live or fixed cells Time-saving - rapid staining, no-lyse/no-wash protocols Convenient - ready-to-use and buffer compatible Reliable - low photobleaching and chemically stable Technical Information CyTRAK Orange™ does not crosslink with or esterify cytoplasmic proteins. CyTRAK Orange™ does the work of two fluorophores. This leaves more opportunity and choice in designing the assay for the cells of interest.