CyGEL™ is a novel thermoreversible gel that is compatible with LIVE cells and organisms. It can be used to immobilise non-adherent cells and beads by simple warming and conversely allowing their recovery by simple cooling.
CyGEL™ is unusual in that it is a liquid when cold and a gel when warmed!
CyGEL™ is available in two sizes; a 8 x 500μl pack which includes 1 x 125μl PBS(40X), or a 20ml bulk value pack for large volume use.
The Science
CyGEL™ is a thermo-reversible hydrogel. Unusually, it is a liquid when cooled and gel when warm. It is compatible with (and non-toxic to) cells, tissues and model organisms. CyGEL™ has been formulated to transition from liquid to gel at ca. 21 °C (i.e. room temperature) allowing immobilization of non-adherent (suspension) cells, beads, micro-tissues, parasites, worms and embryos (such as drosophila), which can be recovered non-destructively by cooling and/or over-dilution using cold buffer.
Why CyGEL™?
There are many reasons why CyGEL™ will help your research:-
IT’S EASY - ready-to-use, straight from the fridge – no powders to dissolve!
IT’S GENTLE - immobilize your living cells or organisms for microscopy
IT’S CLEAR - optically, it’s just like water, with no autofluorescence
IT’S REVERSIBLE - image your precious sample and get it back alive!
CyGEL™ can bring significant benefits to your application:
in MICROPLATE CYTOMETRY:
Immobilize non-adherent and motile objects for 2-D and cytometric analysis
in HIGH CONTENT SCREENING:
Immobilization of non-adherent and fragile 3-D structures in microplates
in FLUORESENCE MICROSCOPY:
Take composite images of motile organisms with confidence and control