Staining solution reagent APOPTRAK™

for researchfor flow cytometryapoptosis

APOPTRAK™ is a chemical derivative of DRAQ5™. It is a novel cellular probe that can enter both INTACT and compromised cells, revealing the relative integrity of an individual cell membrane. APOPTRAK™ is a useful probe for use in apoptosis assays. APOPTRAK™ is available in two sizes; 100μl and 500μl. Why APOPTRAK™? APOPTRAK™ benefits from all the advantageous "far-red" spectral properties of its parent, but differs significantly in its rate of entry into LIVE cells. APOPTRAK™ enters LIVE cells slowly and apoptotic cells rapidly, allowing a positive discrimination between these two populations. Key benefits include:- Different rate of entry into LIVE and apoptotic cells, allowing positive discrimination by flow cytometry (with Annexin V-FITC, for example) Easy to use - no lyse, no wash, no RNase needed Ideal for use with GFP & FITC labels - APOPTRAK™ fluoresces in the far-red region APOPTRAK™ can bring significant benefits to your application: in FLOW CYTOMETRY: positive discrimination between intact cells, membrane-compromised cells, cellular debris validated for use in Annexin V binding assay protocols Technical Information APOPTRAK™ enters LIVE cells slowly and leaky (apoptotic) cells rapidly, to give a signal intensity difference of between 5- and 10-fold, depending on the cell type and treatment. This allows a positive discrimination between these two populations as shown in the example data below. Published data (Wiltshire et al, Cytometry 2000) suggests an ability of APOPTRAK™ to discriminate early and late apoptotic cells when used in combination with Annexin V.