Plasmid purification is an essential step in the transient transfection and cloning workflows. However, typical procedures are manual processes that are time consuming and laborious, and tie up highly skilled staff in routine procedures. Biotage introduce the Biotage PhyPrep, the only automated plasmid purifier capable of operating on the maxi, mega and giga plasmid preparation scales.
Maximise your time
Many researchers are highly skilled and experienced scientists, and their time is valuable. The Biotage PhyPrep frees them from laborious, time-consuming and repetitive manual operations which are vital to the transfection and biomolecule workflows, allowing skilled individuals to focus on valued added activities within the laboratory.
Automation across the scales
The Biotage PhyPrep is capable of purifying maxi, mega and giga scales, delivering 1, 5 or 10 mg of purified plasmid for transfection studies and cloning experiments. Automation of purification steps included filtration of the lysed cells, anion exchange chromatography and washing to deliver pure endotoxin-free material.
Endotoxin free
Following Biotage protocols delivers endotoxin-free* plasmid samples ready for further study with no further sample clean up required.
* With rich media and high copy plasmids
Simple sample preparation
Pre-aliquoted reagents means sample preparation of cell pellets prior to purification is simple and effective. No need to accurately dispense buffers, instead all of the appropriate buffers are supplied in defined amounts ready for immediate use.
System features
Performance by scale
The PhyPrep can operate across three scales of plasmid purification - maxi,