Description
Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is recovery during short 4-minute incubation at 95°C. The activated enzyme possesses the same functionality as Taq DNA polymerase: catalyzes 5'→3' synthesis of DNA, having no detectable 3'→5' proofreading exonuclease activity, but possesses low 5'→3' exonuclease activity. It exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Before activation, the two activities are not detectable.
Product name Cat. No Size Price
Power HotStart Taq DNA Polymerase PR1601 200U Inquiry
Power HotStart Taq DNA Polymerase PR1602 1000U Inquiry